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» La «Polymerase Chain Reaction» ou PCR
Fri 4 Apr - 11:01 by haithem77

» www.cshprotocols.org
Tue 1 Apr - 11:49 by haithem77

» Isolation of Protein from Tissue
Tue 1 Apr - 11:31 by haithem77

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» Les méthodes d’extraction et de purification des ARN
Sat 22 Mar - 13:12 by haithem77

» Culture de cellules
Sat 22 Mar - 11:33 by haithem77

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La «Polymerase Chain Reaction» ou PCR
Fri 4 Apr - 11:00 by haithem77
http://www.ens-lyon.fr/RELIE/PCR/principe/anim/presentation.htm Like a Star @ heaven

http://users.ugent.be/~avierstr/principles/pcr.html Like a Star @ heaven Like a Star @ heaven Like a Star @ heaven

http://www.pcrstation.com/ Like a Star @ heaven Like a Star @ heaven Like a Star @ heaven Like a Star @ heaven

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www.cshprotocols.org
Tue 1 Apr - 11:49 by haithem77
www.cshprotocols.org

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Isolation of Protein from Tissue
Tue 1 Apr - 11:31 by haithem77
Isolation of Protein from Tissue



1. Place the tissues on labeled aluminum foil and immediately place in dry ice. It is imperative that the tissues stay cold so that protease do not have time to act on the protein.
2. Place the tissues in a round bottom tube and add Brij buffer with protease inhibitors added. Add about 1ml Brij/tissues that equals about 100µl in volume. Brij with …

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Forum Molecularstation
Tue 1 Apr - 10:56 by haithem77
www.molecularstation.com

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www.westernblotinfo.com
Tue 1 Apr - 10:44 by haithem77
http://www.westernblotinfo.com

______________________________________________________
Western Blot Protocols
Western Blot Troubleshooting
Western Blot Method
Molecular Biology
Western Blot
----------------------------------------------------------------------------


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Les méthodes d’extraction et de purification des ARN
Sat 22 Mar - 13:12 by haithem77
Annales de Biologie Clinique. Volume 60, Numéro 5, 513-23, Septembre - Octobre 2002, Revues générales

Résumé Summary

Auteur(s) : J.-P. Bastard, S. Chambert, F. Ceppa, M. Coude, E. Grapez, S. Loric, F. Muzeau, F. Spyratos, K. Poirier, V. Copois, C. Tse, T. Bienvenu, Service de biochimie et génétique moléculaire, Hôpital Cochin, 123, boulevard de Port-Royal, 75014 Paris.

Résumé : Les …

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Culture de cellules
Sat 22 Mar - 11:33 by haithem77
Pour tout ce qui est culture cellulaire : http://biowww.net/international/browsefrench-33.html

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Western blotting avec des anticorps biotinylés
Sat 22 Mar - 11:22 by haithem77
[justify]Préparation de l'échantillon:
Rincer les cellules confluantes d'une boite du culture de 60 mm avec du PBS puis effectuer la lyse par addition de 0,5 ml de tampon de lyse bouillant (1% SDS, 10 mM Tris-HCl pH 7,4). Transférer le lysat dans un microtube de 1,5 ml et faire bouillir 5 minutes de plus. Passer aux ultrasons brièvement ou passer plusieurs fois à travers une aiguille de gauge 26 et …[/justify]


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